- Mechanotransduction at the nuclear pore complex
The project will focus on the mechanotransduction at the nuclear envelope level. We aim at understanding if cytoskeleton filaments interact with nuclear proteins and how they can transmit external force no the nucleus affecting the cellular fate. To define the proteins interaction experimental tools will be involved. Starting from Protein production and purification, mass photometry, Calorimetry technique and X-ray crystallography will be used. In addition, to fully characterize protein interaction from mechanical point of view, Molecular dynamic simulations will be implemented. Computational section will be performed at the Mechanobiology lab (departement of chemistry, materials and chemical engineering “Giulio Natta”) and at DEIB department in collaboration with Monica Soncini (https://www.deib.polimi.it/ita/personale/dettagli/370314). Experimental section will be performed at the IEO institute in collaboration with Marina Mapelli (https://www.ieo.it/it/ricerca/People/Researchers/Mapelli-Marina/).
The project is developed in collaboration with Massachusetts Institute of Technology (MIT),Cambridge, MA, USA.
- Modelling cell mechanics by 3D scaffold architecture (one or two students)
This thesis is based at the Mechanobiology lab c/o the Dept. of Chemistry “G. Natta” and the IFN-CNR c/o the Dept. of Physics (Politecnico di Milano – Campus Leonardo). Scaffolds will be fabricated using a micro stereolitography technique called “2-photon laser polymerization”. Cell cultures will be performed using rat bone marrow-derived mesenchymal stem cells and/or cancer cells seeded into the scaffolds; to investigate the mechanotransdcution processes, protein expression and localization (cadherin and lamin a/c) will be investigated by using high resolution fluorescent microscopy. Ideal candidates are those willing to develop knowledge in laser-related materials technology and fluorescent microscopy applied to biological systems.
Tutors: Prof. Emanuela Jacchetti (firstname.lastname@example.org), Prof. Manuela T. Raimondi
- Generation of micro-structured 3D millifluidic chips to model endothelial regeneration (one or two students)
This thesis is based at the Mechanobiology lab c/o the Dept. of Chemistry “G. Natta” and the IFN-CNR c/o the Dept. of Physics (Politecnico di Milano – Campus Leonardo). The work is experimental, it consists in the use of a millifluidic bioreactor to model the endothelial formation. We will analyze the cell adhesion and organization of fibroblast, endothelial cells, and mesenchymal stem cells, undergoes mechanical and chemical stimuli (drugs) to monitor tissue (re)generation. Ideal candidates are those willing to develop knowledge in laser-related materials technology and fluorescence microscopy.